Fig. 6.

Hmga2 is necessary for the pioneering activity of Otx2 in the engagement of new enhancers during the transition from embryonic stem cells (ESCs) into epiblast-like stem cells (EpiLCs). a Otx2 association with the indicated genes upon Hmga2 silencing. ESCs transfected with si Ctrl or si Hmga2 were induced to differentiate into EpiLCs and the Otx2 binding to the enhancers of indicated genes was measured by ChIP-qPCR. Data are presented as mean ± SEM of independent biological replicates (Plekha1, FGF5, Hells, n = 3; Slc16a3, Myrf, n = 4). *P <0.05, **P <0.01. b ChIP-qPCR analysis of the binding of Otx2 to the enhancers of the indicated genes in EpiLCs derived from wt and Hmga2 KO induced pluripotent stem cell (iPSC) clones. qPCR data represent mean of two independent experiments (n = 2) ± SD for each of the indicated wt or Hmga2 KO iPSC clones. c Otx2 mRNA and protein expression levels in wt and KO iPSCs induced to differentiate into EpiLCs. qPCR data are means of three independent experiments (n = 3) ± SD