Fig. 3.

CYP2E1 content and activity in wild-type and Has3−/− mice. a Representative CYP2E1 immunoblot using samples from olive oil-treated wild-type and Has3−/− mice. GAPDH was used as a loading control. b Semi-quantification of hepatic CYP2E1 content after normalization to GAPDH. Data are expressed as fold change over wild-type mice (n = 6 each genotype). c Hepatic CYP2E1 activity assay in microsomes isolated from the wild-type and Has3−/− mice at baseline (before CCl₄ exposure) (n = 6 mice per experimental group). The data are expressed as fold change over wild-type. Pos cntl, microsomes prepared from a single mouse fed an ethanol-containing diet for 5 weeks (ethanol is known to increase CYP2E1). Neg cntl, microsomes prepared from a single mouse exposed to CCl₄ and euthanized 24 h later (CCl₄ consumptively depletes CYP2E1)