Table 1.
Proportion of | Number of | ||||||||
---|---|---|---|---|---|---|---|---|---|
Nerve vertical | Muscle vertical | nerve–muscle | nerve–muscle | ||||||
Expt | Experiment | Align‐ment | separation from | separation from | Lowest activation | preparations | preparations | Likely | Figure or |
No. | description | with device axis | device (mm) | device (mm) | frequency (Hz) | activated | tested (n) | activation site | videoa |
1 | Nerve spanning centre, muscle low | Perpendicular | 0.7 | 0.7 | 260 | 0.5 | 2 | Uncertain, either in muscle over y ≤ −20 mm or in nerve spanning 10 mm ≤ y ≤ 25 mm | 4A, 4B |
2 | Nerve spanning centre, muscle high | Perpendicular | 0.7 | ≥8 | 240 | 0.5 | 2 | Nerve, either at ligated end or where rising over y ≤ −25 mm or where straight over −25 mm ≤ y ≤ −10 mm | |
3 | Nerve ligated end at centre, muscle high | Perpendicular | 0.7 | ≥8 | 320 | 1 | 1 | Nerve, either at ligated end or where rising over y ≤ −29 mm or where straight over −25 mm ≤ y ≤ −10 mm | |
4 | Nerve spanning centre, muscle low | Parallel | 0.7 | 0.7 | None | 0 | 2 | None | |
5 | Nerve spanning centre, muscle high | Parallel | 0.7 | ≥8 | 790 | 0.33 | 3 | Nerve, either at ligated end or where rising over z ≤ −10 mm or at centre z = 0 | 4C |
6 | Nerve ligated end at centre, muscle high | Parallel | 0.7 | ≥8 | 770 | 1 | 1 | Nerve, either near centre z = 0 or where rising from z ≤ −32 mm | |
7 | Nerve spanning centre, muscle high | 45 deg to axis | 0.7 | 8 | 440 | 1 | 1 | Nerve | |
8 | Muscle at centre | Parallel | 6 | 0.7 | 180 | 1 | 2 | Muscle | 4D, S1 |
9 | Muscle at centre, bath raised | Parallel | ≥4.8 | 4.8 | 590 | 1 | 1 | Muscle | S2 |
10 | Muscle at centre | Perpendicular | 6 | 0.7 | 480 | 1 | 2 | Muscle | |
11 | Muscle at centre as in Expt 6, d‐tubocurarine blocking nerve‐evoked activation | Parallel | 9 | 0.7 | 460 | 1 | 1 | Muscle | |
12 | Nerve wrapped one half‐turn around 2.9 mm diameter pillar at device centre | Perpendicular | 1.5 | 8 | 230 | 1 | 1 | Nerve bend | 4E, S3 |
13 | Nerve wrapped one half‐turn around 2.9 mm diameter pillar at device centre | Parallel | 1.5 | ≥8 | 480 | 1 | 3 | Nerve bend | |
14 | Nerve wrapped one half‐turn around 2.9 mm diameter pillar at z = 18 mm | Parallel | 0.7 | 11 | 250 (though slight twitching at 190) | 0.67 | 3 (includes 1 in Expt 19) | Nerve bend | |
15 | Nerve wrapped one half‐turn around 5.0 mm diameter pillar at z = 18 mm | Parallel | 0.7–2 | 11 | 360 | 1 | 1 | Nerve bend | |
16 | Nerve wrapped one half‐turn around 8.5 mm diameter pillar at z = 18 mm. | Parallel | 2 | 11 | 350 | 1 | 2 | Nerve bend | |
17 | Nerve electrically activated at ligated end; nerve spanning centre, muscle raised | Parallel | ≥1 | ≥5.4 | Electrical activation not blocked to 940 Hz | 0 | 2 | None | |
18 | Nerve electrically activated at ligated end; nerve spanning centre, muscle raised | Perpendicular | ≥1 | 5.4 | Electrical activation not blocked to 930 Hz | 0 | 1 | None | |
19 | Nerve electrically activated at ligated end; nerve wrapped one half‐turn around 2.9 mm diameter pillar at z = 18 mm | Parallel | 1.5 | 5.4 | 730, with electrical activation not blocked | 1 | 1 | Nerve bend | 4F |
Videos (S1–S3) can be found in the online Supporting information.