Fig. 2.

Ovarian kiss1 and kiss1r expression in relatively reproductive-aged and older mice. Six mice on normal chow or six mice on high-fat diet had oophorectomy at 20 weeks (reproductive-aged group). The remaining six mice in the NC group continued NC while the remaining six on HF diet were converted to NC and both groups had oophorectomy at 32 weeks (older group) of age. Kiss1 and kiss1r mRNA expression levels were significantly higher in older (n = 10) compared to reproductive-aged mice (n = 8). Diet-induced weight gain did not affect kiss1 or kiss1r mRNA expression levels. *p < 0.05