Fig. 5.

Stratification by age of kiss1r mRNA expression levels in cumulus granulosa cells (GCs) collected from women who underwent in vitro fertilization. Cumulus GCs were collected by mechanically cutting the cumulus cell layer from each oocyte following oocyte retrieval. Women aged 35 (n = 4) had significantly lower kiss1r mRNA expression level compared to women aged between 35 and 39 (n = 6) who had significantly lower kiss1r mRNA expression levels compared to women aged ≥ 40 (n = 2). Data are described as median (range). a p < 0.05 compared to <35; b p < 0.05 compared to 35–39