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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Clin Cancer Res. 2015 Dec 11;22(7):1800–1812. doi: 10.1158/1078-0432.CCR-15-0780

Figure 2.

Figure 2

EMT regulator Snail is required for TNF-α-mediated EMT in HCC cells. A, Western blot analysis of several EMT regulators in HCC cells treated with TNF-α (10 ng/ml) at different time points. B, morphological changes of Hep3B and Tong Snail knockdown stable cells with or without TNF-α stimulation (10 ng/ml for 72 hours). C, Western blot analysis of the Snail and several EMT markers from treated cells as described in (B). D, migration analysis of Hep3B and Tong Snail knockdown stable cells after TNF-α (10 ng/ml) stimulation for 48 hours. *p < 0.05. **p < 0.001 compared with mock. E, invasion analysis of Hep3B and Tong Snail knockdown stable cells after TNF-α (10 ng/ml) stimulation for 72 hours. *p < 0.05. **p < 0.001 compared with mock.