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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Jan 26;1859(4):553–563. doi: 10.1016/j.bbagrm.2016.01.005

Figure 4. Transcription activation by the ACTB UAS is unidirectional on TATA but bidirectional on a composite TATA/INR core promoter.

Figure 4

The top part shows the luciferase reporters containing the UAS of ACTB (−120 to −40 bp) inserted upstream of heterologous TATA, TATA/INR or INR core promoters in forward or reverse orientation. The lower part shows the luciferase activities of the above reporters in transfected HEK293 cells. Luciferase activities are the means of three independent experiments. Primer extension analyses of correctly initiated Luc transcripts (+1), including an internal control (ctrl) transcript (as in Figs. 2 and 3), are shown at the bottom.