Skip to main content
. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Jan 26;1859(4):553–563. doi: 10.1016/j.bbagrm.2016.01.005

Figure 6. ACTB promoter activity requires SRF but not NFY or SP1.

Figure 6

A. Relative luciferase activity from the natural ACTB promoter-luciferase construct (see Fig. 1A) in cells transfected with control (Ctrl), SRF, NFYA, NFYB or SP1 siRNAs. Luciferase activity was normalized to total protein. Luciferase activity in cells transfected with the siRNA Ctrl was arbitrarily set to 1. Results are the means from three independent experiments. Asterisks indicate significant differences (P<0.05) relative to Ctrl.

B. Western blot analysis of SRF, NFYA, NFYB and SP1 proteins in extracts of cells transfected with the control siRNA (si-Ctrl) and specific siRNAs as indicated.