Fig. 2.
A dramatic postmortem 2-AG increase is prevented by MW but not by fast freezing of intact head in liquid nitrogen
Brain samples were analyzed from fixed with microwave irradiation brains (MW), from non-MW brains frozen within 30 sec after removing from the heads (non-MW), or from non-MW brains frozen in situ in the animal heads in liquid nitrogen within 3 sec after decapitation (non-MW, fast frozen). Samples were analyzed using UPLC-MS/MS analysis as described in the Methods. Values (also indicated above the bars) are mean ± SD, n=4. * - Statistically different (p<0.05) as compared to MW using ANOVA with Tukey post-hoc test. The data is representative of two independent sets of experiments.
