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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Lipids. 2016 Mar 28;51(4):487–495. doi: 10.1007/s11745-016-4144-y

Fig. 3.

Fig. 3

2-AG is rapidly produced and hydrolyzed upon incubation of non-MW brain tissue

Frozen non-microwaved brains were pulverized and 10 mg of brain powder was spiked with 2-AGd8 (10 µg). 2-AG and 2-AGd8 were extracted immediately or after incubation at 40°C for 30 min and quantified using UPLC-MS/MS analysis as described in the Methods. Values (also indicated above the bars) are Mean ± SD, n=4. Because labeled 2-AGd8 was used to account for hydrolysis of exogenous 2-AG, we could not use it as an internal standard. Thus, the results are reported as peak area for 2-AGd8 and unlabeled 2-AG.* - Statistically different (p<0.05).