. Author manuscript; available in PMC: 2017 Apr 1.

Published in final edited form as: Lipids. 2016 Mar 28;51(4):487–495. doi: 10.1007/s11745-016-4144-y

Table 1.

Exogenous and endogenous 2-AG stability in biological matrix upon exposure to microwave irradiation

	Exogenous 2-AGd₈ recovery upon MW, %			Endogenous 2-AG, ng/gww
	AV	SD	Significance	AV	SD	Significance
MW+infusion+MW	102.1	22.7	N/S
MW				91.9	13.4
MW+MW				85.8	8.4	N/S

To validate stability of exogenous 2-AG upon exposure to head-focused microwave irradiation in biological matrix, 2-AGd₈ was infused stereotexically (i.c.i., 10 ug/animal) into microwaved brain. 2-AGd₈ was extracted 10 min after infusion (MW+infusion) or 10 min after re-exposure to microwave irradiation to validate 2-AG stability (MW+infusion+MW). The 2-AGd₈ recovery upon MW was calculated as a ratio of peak areas between MW+infusion+MW and MW+infusion. Statistical significance was calculated for peak areas (data not shown). The stability of endogenous 2-AG was validated by comparison of 2-AG levels in microwaved tissue before (MW) and after (MW+MW) second exposure to MW irradiation. Abbreviations are: AV-average; SD-standard deviation; N/S – not statistically different, n=4.