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. 2016 Feb 19;35(7):706–723. doi: 10.15252/embj.201592759

Figure EV5. AUG context and translation.

Figure EV5

  • A
    Cumulative plots show the distribution of initiation context scores in zebrafish for uORFs, oORFs, 3′ UTR ORFs (dORFs), and CDS, further broken down by their translation classification. The TLS background is calculated by scoring the nucleotide context around 50,000 randomly selected TLS positions.
  • B
    uORF AUG scores are significantly correlated with uORF translation in single‐uORF transcripts at 5 hpf in zebrafish (Pearson's r = 0.30, P < 2.2e‐16).
  • C
    uORF AUG scores are inversely correlated with downstream CDS translation in single‐uORF transcripts (Pearson's r = −0.094, P = 5.15e‐5).
  • D, E
    Cumulative plots show the distribution of initiation context scores in (D) mouse and (E) human, for uORFs, oORFs, 3′ UTR ORFs (dORFs), and CDS. The TLS background is calculated by scoring the nucleotide context around 50,000 randomly selected TLS positions in each species.