Figure EV1. Avil‐Cre::Ret^{+/ eGFP} DRG cell sorting strategy.

Hierarchical gating strategy for the isolation of live Ret⁺ sensory neurons. DRG cells were isolated from Avil‐Cre::Ret^{+/ eGFP} mice, quantified, and selected according to their size and complexity. Dead cells and immune cells were excluded using the living dye Sytox Blue and an antibody against CD45. Live sensory neurons were defined by their levels of eGFP fluorescence and IB4 binding. The bottom left plot is identical to that in Fig 2A.