Figure 2. Taz and Tead family proteins are not required for the self‐renewal of J1 mouse ES cells (see also Fig EV4).

• A
  Colony morphology and AP activity of ES cells upon KD of Yap1 and Taz.
• B–D
  mRNA expression levels of Yap1 and Taz (B), Pou5f1, Nanog, Sox2, and Esrrb (C), and lineage‐specific marker genes (D) upon KD of Yap1 and Taz. Data are represented as mean ± SD.
• E
  Colony morphology and AP activity of ES cells upon KD of Tead1/Tead3/Tead4.
• F, G
  mRNA expression levels of Tead1, Tead3, and Tead4 (F) and Pou5f1, Nanog, Sox2, and Esrrb (G) upon KD of Tead 1/3/4. Data are represented as mean ± SD.
• H
  Protein levels of Tead1, Tead3, Tead4, and Pou5f1 upon KD of Tead1/Tead3/Tead4. N.S., non‐specific.
• I
  mRNA expression levels of lineage‐specific marker genes upon KD of Tead1/Tead3/Tead4. Data are represented as mean ± SD.