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. 2016 Feb 25;17(4):519–529. doi: 10.15252/embr.201540933

Figure EV2. Yap1 is dispensable for self‐renewal of E14 and CJ7 mouse ES cells (related to Fig 1).

Figure EV2

  • A–C
    Data from CJ7 ES cells. Colony morphology and AP activity of Yap1 KD cells (A), mRNA levels of Pou5f1, Nanog, Sox2, and Esrrb in Yap1 KD cells (B), and protein expression levels of Yap1, Pou5f1, and Gapdh were measured in Yap1 KD cells (C). mRNA level data are represented as mean ± SD.
  • D–F
    Data from E14 ES cells. Colony morphology and AP activity of Yap1 KD cells (D), mRNA levels of Pou5f1, Nanog, Sox2, and Esrrb in Yap1 KD cells (E), and protein expression levels of Yap1, Pou5f1, and Gapdh were measured in Yap1 KD cells (F). mRNA level data are represented as mean ± SD.
  • G–I
    Data from CJ7 ES cells (ESC). Colony morphology and AP activity of Yap1 KO clones (G), mRNA levels of Pou5f1, Nanog, Sox2, and Esrrb in Yap1 KO clones (H), and protein expression levels of Yap1, Pou5f1, and Gapdh were measured in Yap1 KO clones (I). mRNA level data are represented as mean ± SD.
  • J–L
    Data from E14 ES cells. Colony morphology and AP activity of Yap1 KO clones (J), mRNA levels of Pou5f1, Nanog, Sox2, and Esrrb in Yap1 KO clones (K), and protein expression levels of Yap1, Pou5f1, and Gapdh were measured in Yap1 KO clones (L). mRNA level data are represented as mean ± SD.