Table 2.
List of differentially expressed proteins identified by MALDI-TOF/MS in C. annuum leaves.
| Spot number | Accession number | Nominal mass (M r) |
Theoretical pI/experimental pI | Protein identification | Species | Sequence coverage (%) |
MASCOT Score |
|---|---|---|---|---|---|---|---|
| 1 | XP_004249273 | 55408 | 7.5/4.2 | Adenylosuccinate synthetase, chloroplastic | Solanum lycopersicum | 25 | 51 |
| 2 | XP_008793948 | 44084 | 8.2/4.1 | E3 ubiquitin-protein ligase PUB23-like | Phoenix dactylifera | 20 | 56 |
| 3 | XP_010243911 | 57603 | 5.0/4.8 | Zinc finger protein 160-like | Nelumbo nucifera | 17 | 53 |
| 4 | XP_010655136 | 78992 | 6.4/6.4 | Vacuolar protein sorting-associated protein 53 A isoform X2 | Vitis vinifera | 16 | 66 |
| 5 | AHL68475 | 27519 | 6.7/5.0 | Ribulose-1,5-bisphosphate carboxylase/oxygenase, partial (chloroplast) | Androcymbium cf. capense Chacon 20 | 41 | 47 |
| 6 | KHG29665 | 13451 | 11.4/5.0 | tRNA-specific-2-thiouridylase mnmA | Gossypium arboreum | 50 | 42 |
| 7 | XP_009393059 | 42588 | 6.1/5.1 | GDP-mannose 3,5-epimerase 2-like | Musa acuminata subsp. malaccensis | 32 | 46 |
| 8 | XP_003058724 | 59677 | 9.1/4.6 | Nucleoporin-like protein | Micromonas pusilla CCMP1545 | 33 | 43 |
| 9 | XP_006647478 | 17489 | 4.2/4.8 | Probable calcium-binding protein CML17-like | Oryza brachyantha | 46 | 63 |
| 10 | EMT25898 | 18876 | 8.5/4.6 | Ribulose bisphosphate carboxylase small chain PWS4, chloroplastic | Aegilops tauschii | 80 | 44 |
| 11 | EMS51364 | 17984 | 8.9/4.3 | DEMETER-like protein 2 | Triticum urartu | 50 | 69 |
| 12 | XP_009398204 | 24720 | 9.5/5.1 | Oxygen-evolving enhancer protein 3-1, chloroplastic-like | Musa acuminata subsp. malaccensis | 52 | 64 |
| 13 | XP_010517956 | 20595 | 6.5/4.8 | Molybdopterin synthase catalytic subunit-like | Camelina sativa | 80 | 62 |
| 14 | XP_008677250 | 28739 | 8.8/5.4 | MADS-box transcription factor 26 isoform X2 | Zea mays | 42 | 51 |
| 15 | XP_010046336 | 55906 | 5.3/7.0 | Disease resistance protein RPS2-like | Eucalyptus grandis | 19 | 45 |
| 16 | XP_004951624 | 13105 | 5.6/6.2 | Mediator of RNA polymerase II transcription subunit 11-like | Setaria italica | 59 | 48 |
| 17 | AFB70663 | 15211 | 11.8/4.7 | Ribosomal protein L16, partial (chloroplastic) | Weingartia kargliana | 63 | 52 |
| 18 | AEJ73201 | 5179 | 6.2/7.0 | CIN-like protein, partial | Nandina domestica | 53 | 43 |
| 19 | XP_009588622 | 33538 | 9.2/4.4 | Mitochondrial calcium uniporter regulatory subunit MCUb-like isoform | Nicotiana tomentosiformis | 26 | 46 |
| 20 | XP_004979712 | 107060 | 6.3/5.5 | Putative disease resistance RPP13-like protein 3-like | Setaria italica | 14 | 52 |
| 21 | ABS87384 | 56157 | 5.0/4.9 | Pyruvate kinase | Lactuca sativa | 20 | 56 |
| 22 | BAF01042 | 57010 | 8.7/4.8 | Receptor-like protein kinase | Arabidopsis thaliana | 41 | 50 |
| 23 | KIY92373 | 29267 | 8.7/4.4 | Phosphoglycerate kinase, partial | Monoraphidium neglectum | 51 | 54 |
| 24 | AAR08850 | 4803 | 9.4/5.2 | Resistance protein candidate | Vitis amurensis | 100 | 55 |
| 25 | AAL83898 | 10928 | 11.6/6.9 | Beta-keto acyl reductase | Zea mays | 87 | 45 |
| 26 | AAF72909 | 18001 | 5.4/7.0 | Resistance gene analog protein | Medicago sativa | 56 | 38 |
| 27 | AIF71068 | 21906 | 8.6/5.8 | ATP synthase CF1 alpha subunit, partial (chloroplast) | Actiniopteris semiflabellata | 49 | 44 |
| 28 | CAC87838 | 26888 | 5.0/4.5 | Cullin 1D | Nicotiana tabacum | 25 | 53 |
| 29 | XP_009147561 | 34297 | 9.4/7.0 | F-box only protein 8-like | Brassica rapa | 33 | 53 |
| 30 | XP_012064817 | 54515 | 8.7/5.7 | Double-stranded RNA-binding protein 2 | Jatropha curcas | 14 | 57 |
| 31 | XP_006450493 | 45127 | 6.0/4.4 | Hypothetical protein CICLE_v10010442mg, partial | Citrus clementina | 19 | 43 |
| 32 | Q2RAX3 | 52023 | 7.1/7.0 | CBL-interacting protein kinase 33 | Oryza sativa japonica group | 13 | 47 |
| 33 | XP_009588623 | 22039 | 8.3/6.4 | Uncharacterized protein LOC104086136 isoform X2 | Nicotiana tomentosiformis | 39 | 54 |
| 34 | BAB40826 | 16893 | 7.9/6.3 | Reverse transcriptase | Zea mays | 30 | 46 |
| 35 | KHG25806 | 41699 | 8.9/4.4 | Eukaryotic translation initiation factor 3 subunit D | Gossypium arboreum | 26 | 50 |
| 36 | AIU48281 | 79429 | 7.5/6.0 | Minichromosome maintenance 5 protein, partial | Illicium henryi | 27 | 50 |
| 37 | AKL82244 | 11565 | 8.1/7.0 | Glyceraldehyde 3-phosphate dehydrogenase, partial | Rosa soulieana | 84 | 52 |
| 38 | KHN40347 | 11468 | 5.6/5.4 | Putative caffeoyl-CoA O-methyltransferase | Glycine soja | 37 | 63 |
| 39 | XP_006485364 | 19263 | 8.9/6.6 | Ras-related protein RABH1b-like isoform X3 | Citrus sinensis | 31 | 47 |
| 40 | XP_003598507 | 43923 | 6.8/7.0 | F-box/kelch-repeat protein | Medicago truncatula | 22 | 47 |