Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 23;7(4):236–249. doi: 10.1007/s13238-016-0254-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

PMC Copyright notice

RT-qPCR verification of the RNA-Seq results. The 8 randomly selected genes (TTHERM_00961910; TTHERM_00730300; TTHERM_00426250; TTHERM_00077290; TTHERM_00865200; TTHERM_00338290; TTHERM_01109940; TTHERM_00763040) plus CYC2 TTHERM_00079530) and SPO11 (TTHERM_00627090) were performed with RT-qPCR. The primers were designed to across an intron to eliminate potential confluence from residue genomic DNA contamination. cDNA templates were from of 3 independent biological repeats of cell samples respectively at 2 h (A), 2.5 h (B), 3 h (C), and 3.5 h (D) after conjugation initiation. The tendencies of mRNA expression changes were in accordance with the RNA-Seq analysis results, which well-validated of the sequencing data