Table 5.
Oligonucleotide primers used in this study
| Primer | Resultant construct | Sequence (5′-3′) |
|---|---|---|
| BamINLB | pBYM3, pBYM4 and pBINU1 | CGGGATCCTCAATTTGTTTACACCAC |
| BamINRB | pBYM3, pBYM4 and pBINU1 | CGGGATCCCAGTTTAAACTATCAGTG |
| SRS2Fw | pSRS2 | GGAATTCCAGGAGTGAAGACATCTGC |
| SRS2Rv | pSRS2 | GGAATTCCGACTTGGGACTATTGGAC |
| RAD52Fw | pRAD52 | GGAATTCTAACGGTGAGTGTGGCAACG |
| RAD52Rv | pRAD52 | GGAATTCTGAACCTAAGGATTCCGCTG |
| SMI1Fw | pSMI1 | GGAATTCCACTGATCCATTTACCTGC |
| SMI1Rv | pSMI1 | GGAATTCCTCATGCTCTTCAATGTCG |
| ERG28_2_Fw | pERG28 | CGAGGAATTCTCGGGGACAACAACTTCAG |
| ERG28Rv | pERG28 | GGAATTCTGAGCTGGAGCAGACATTG |
| URA3-BamHIfw | pBINU1and pBINU2 | CGGGATCCCGAGTCGCATAAGGGAGAGC |
| URA3-BamHIrv | pBINU1 | CGGGATCCCGATCAGCGTGGTCGTGAAG |
| URA3-EcoRI | pBINU2 | CGGAATTCCTGAAGCTCTAATTTGTG |
| URA3-probe-Fw | URA3 probe | CTTAACCCAACTGCACAGAACA |
| URA3-probe-Rv | URA3 probe | GCAATAAAGCCGATAACA |
| Amp-probe-Fw | Amp r gene probe | TGCAATGATACCGCGAGAC |
| Amp-probe-Rv | Amp r gene probe | CGAACTGGATCTCAACAGCGGTAA |
| pBY1-RB-Seq | for sequencing | GAACGCGCAATAATGGTTTCTG |
| pBY1-RB-Seq2 | for sequencing | GGGCGCACCGCAGATGGAAA |