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Primers used in real time PCR
| Gene | Primer | Primer sequence | Size of PCR product |
|---|---|---|---|
| gp85 | Pf | GTCACTAATGTTACTGCTTGCG | 434 bp |
| Pr | GAACTACACCAATTAGAAAG | ||
| chNHE1 | Pf | CTGAACAAACAGCACCACAAC | 291 bp |
| Pr | GAGGAGGAAGAGGAAGAAGATG | ||
| GAPDH | Pf | GGTGGTGCTAAGCGTGTTA | 179 bp |
| Pr | CCCTCCACAATGCCAA |
The gp85 primers were used for detecting the mRNA level expression of ALV-J
