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. 2016 Apr 2;16:77. doi: 10.1186/s12870-016-0766-8

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© Salleh et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — ROS levels and activity of ROS-related enzymes in wallflower petals (a) Quantification of relative H₂O₂ production in wallflower petals (Stage 1- first fully open flower to Stage 6 – abscission; mean ± SE, n = 9); (b) Quantification of relative H₂O₂ production in detached wallflower petals in the first 3 days following STS and 6-MP treatment. (mean ± SE, n = 9). c zymograms of APX (ascorbate peroxidase), SOD (superoxide dismutase) and CAT (catalase) isoforms active in wallflower petals at Stages 1–6. Loading control gel images are shown beneath each zymogram; (d) quantification of zymogram bands using Image-J (arbitrary units)