Table 2.
Affinity and Kinetic Data for PPARγ Interactionsa
| compound | ka (1/M·s) | kd (1/s) | KD (M) | χ2 (RU2) |
|---|---|---|---|---|
| Rosi: Affinity | 1.39 × 10−5 (±1.1 × 10−6) | 0.911 (±0.46) | ||
| SR2067: Kinetics | 3.17 × 104 (±3.9 × 102) | 1.63 × 10−2 (±1.6 × 10−2) | 5.13 × 10−7 (±3.2 × 10−8) | 0.368 (±0.17) |
| SR2067: Affinity | 5.65 × 10−7 (±1.8 × 10−8) | 0.327 (±0.07) |
a
Due to the fast interaction between Rosi and PPARγ, no rate constants could be calculated. For the SR2067 interaction, both kinetic and steady-state affinity analyses were performed. Numbers in parentheses indicate the standard errors calculated from triplicate experiments.