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Establishment and immunophenotyping of F3.ChAT human neural stem cell line. (a) A phase contrast image of F3.ChAT cells. (b) Immunocytochemical identification of nestin protein (green) in F3.ChAT cells. (c) Immunocytochemical identification of choline acetyltransferase (ChAT) protein (red) in F3.ChAT cells. (d) Merge of nestin- (b) and ChAT-positive (c) images.
