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. 2016 Mar 2;148(1):70–82. doi: 10.1111/imm.12588

Figure 6.

Figure 6

Expression of NKG2D and tumour necrosis factor‐α (TNF‐α) in gestational day (GD) 9·5 uterine natural killer (uNK) cells treated with tumour necrosis factor‐like weak inducer of apoptosis (TWEAK) or a TWEAK‐specific antibody. Uterine NK cells were isolated from a GD 9·5 normal pregnant mouse and incubated with recombinant TWEAK or a TWEAK‐specific antibody at the indicated final concentrations for 24 hr. The levels of NKG2D (a) and TNF‐α (b) protein in the uNK cells were analysed by Western blotting. Representative blots are shown in the upper panel and the histograms in the lower panel show the results of densitometric analyses of NKG2D or TNF‐α expression (normalized to β‐actin to correct for protein loading). The data represent the means ± SEM from at least three independent experiments. Columns with different superscript letters are significantly different (P < 0·05).