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. Author manuscript; available in PMC: 2016 Apr 4.
Published in final edited form as: Biochemistry. 2016 Feb 1;55(5):809–819. doi: 10.1021/acs.biochem.5b01254

Figure 3.

Figure 3

Charactarization of wild-type and Lys700Ala pUL15C. Differential scanning fluorimetry analysis of (a) wild-type and (b) mutant enzymes in the absence (gray trace) and presence of 20 μM β-thujaplicinol (black trace). (c) Duplex DNA cleavage activity and kinetic analysis of pUL15C using the dual-probe fluorescence assay. Enzyme activity was determined at protein concentrations of 0.6 μM (columns 1 and 3) and 0.3 μM (columns 2 and 4). Km, kcat, and kcat/Km values for both are provided in the inset.