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. 2016 Apr 4;6:23969. doi: 10.1038/srep23969

Figure 5. Levels of synaptophysin 1 in Notch conditional knockout mouse brains.

Figure 5

(a) The representative hippocampal images of adult neuron-specific Notch1/Notch2 double-knockout mice (N1N2DcKO), Notch2 knockout mice (N2cKO) and control mice. Sagittal sections of 23–28 week-old mice brains were immunostained for EYFP (green) and synaptophysin 1 (syp, red). Inactivation of Notch1 and Notch2 genes were achieved by endogenous tau promoter-driven CrePR protein. EYFP signals indicate neuronal cells with successful recombination at Rosa26stopfloxYFP allele. Scale bar, 200 μm. (b) Magnified images of CA1 region in (a). Scale bar, 100 μm. (c) Quantitative analysis of synaptophysin 1 immunoreactivity. The immunofluorescence signals of CA1 were calculated (n = 4 mice each, means ± SEM, *p < 0.05 by Tukey’s test).