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. 2016 Apr 4;6:23969. doi: 10.1038/srep23969

Figure 7. PKG inhibitor repressed Jagged1-induced increase in synaptophysin 1.

Figure 7

(a) Representative immnoblot images of rat primary neuronal culture cocultured with NIH3T3 cells for synaptophysin 1 (syp), β-III-tubulin (βIIItub) and Jagged1 (Jag1). Coculture was maintained for 18 hours with or without several inhibitors: Rp-cAMPS triethylamine (#1), H-9 dihydrochloride (#2) and Ro 20-1724 (#3) (representative data from 4 experiments). Jag1-induced increase in the levels of synaptophysin 1 was blocked by H-9 dihydrochloride. (b) Representative immunoblot images for synaptophysin 1 (syp), β-III-tubulin (βIIItub) and Notch1 NICD in the coculture of murine primary neuronal culture and NIH3T3 cells. DAPT or KT5823 is treated at 10 μM. The gels have been run under the same experimental conditions, and representative blots are cropped and used in this figure. Full-length gels are presented in Supplementary Fig. S4. (c) Relative protein levels of synaptophysin 1 (syp) quantified from the immunoblot analyses of the coculture with Jag1-expression NIH3T3 cells (n = 4, means ± SEM, *P < 0.05 by Steel-Dwass test).