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. 2016 Apr 4;6:24060. doi: 10.1038/srep24060

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Copyright © 2016, Macmillan Publishers Limited

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Nitric oxide synthase 1 activity, levels, and its relationship with LV function. Nitric oxide synthase 1 activity, levels, and its relationship with LV function. (a) GTP cyclohydrolase 1 (GCH1) protein levels. (b) xanthine oxidoreductase (XOR) protein levels. (c) Total NOS and NOS1 activity in myocardial tissue from non-failing and ischemic hearts. (d) NOS1 protein levels. Dimeric and monomeric NOS1 determination. Values from the CNT group were set to 100. The data are expressed in optical density, arbitrary units (au). The values are normalized to GAPDH and finally to the CNT group. In the boxplots with dots the middle line in box represents the median, lower box bound the first quartile, upper box bound the third quartile, whiskers the 95% confidence interval of the mean. (e) Correlation between NOS1 protein levels and left ventricular ejection fraction (LVEF) in ICM patients. *P < 0.05 versus the CNT group.