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. 2016 Apr 4;6:23942. doi: 10.1038/srep23942

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(A) NK cells form clusters when cultured either in the presence of recombinant IL-2 (rIL-2) or activated CD4⁺ T cells in a trans-well assay. (B) The histograms represent the percentage of CD300c⁺ CD56^bright NK cells cultured under several conditions in the transwell assay as described in Materials and Methods. (C) Bar graph represents the MFI of CD300c expression on CD56^bright NK cells cultured under the indicated conditions in the transwell assay. The data are from independent experiments from 4 donors. The error bars indicate the average ± SEM. rCD4 T-cells: resting CD4+ T cells; aCD4 T-cells: activated CD4+ T cells; α-IL-2: neutralizing anti-IL-2 mAb; rec IL-2: recombinant IL-2.