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. 2016 Apr 4;6:23942. doi: 10.1038/srep23942

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Copyright © 2016, Macmillan Publishers Limited

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(A) Binding of the purified fusion proteins CD300a-Ig (top graph) and CD300c-Ig (bottom graph) to dead Jurkat cells. Increasing concentrations of fluorochrome labeled CD300a-Ig and CD300c-Ig proteins were incubated with dead Jurkat cells and then acquired in a flow cytometer. LAIR1 R65K-Ig fusion protein served as negative control. Graphs represent the binding (MFI) of the fusion proteins to dead cells. The data are representative of two independent experiments. (B) ELISA assay shows binding of increasing concentrations of CD300a-Ig (top graph) and CD300c-Ig (bottom graph) fusion proteins to pure lipids that are coated on plates. LAIR1 R65K-Ig fusion protein served as negative control. The data is a representative of 2 independent experiments. (C) Binding of CD300-Ig fusion proteins to liposomes. Liposomes of specified compositions were prepared and coupled to a L1 biosensor. The binding of CD300a-Ig (top) and CD300c-Ig (bottom) was analyzed by allowing the proteins to pass through the L1 sensor. The curves represent the binding of fusion proteins to liposome coated L1 chips. (D) The binding (RU) for plateau values is shown in the bar graph and the error bars represent the average ± SEM. LAIR1 R65K-Ig fusion protein served as negative control. Results shown are from 3 independent experiments.