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. 2016 Mar 31;3(2):ENEURO.0116-15.2016. doi: 10.1523/ENEURO.0116-15.2016

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Copyright © 2016 Soumier et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 2. — Examples of immunohistochemical markers. A, After kainate injection, all mature granule neurons and some BrdU-labeled 16-d-old NeuN⁺ neurons expressed zif268, indicating synaptic activation. GCL, granule cell layer B, Dividing cells (arrows) were identified using PCNA immunohistochemistry. C, Cells surviving 2-3 weeks (arrows) were identified with BrdU immunohistochemistry (gray-brown); immunonegative cells were stained with blue-purple counterstain.