. Author manuscript; available in PMC: 2016 Oct 5.

Published in final edited form as: Angew Chem Int Ed Engl. 2015 Aug 25;54(41):12064–12068. doi: 10.1002/anie.201506346

Table 1.

Optogenetic Bax constructs and their subcellular location.

Construct	Dark	Dark + STS^[a]	Mitochondrial Half-Life^[b]
Cry2-mCh-Bax^WT	Mitochondrial + Cytoplasmic	Mitochondrial	200 ± 10 s
Cry2-mCh-Bax^S184E	Cytoplasmic	Mitochondrial	210 ± 10 s
Cry2-mCh-Bax^S184V	Mitochondrial	Mitochondrial	N.D. ^[c]
Bax^WT-Cry2-mCh	Cytoplasmic	Cytoplasmic	130 ± 10 s
Bax^S184E-Cry2-mCh	Cytoplasmic	Cytoplasmic	130 ± 10 s
Bax^S184V-Cry2-mCh	Cytoplasmic	Cytoplasmic	N.D.

^[a]

STS = staurosporine.

^[b]

Mitochondrial residence time following a single 100 ms 488 nm light pulse,

^[c]

N.D. = Not determined.