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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Neurobiol Dis. 2015 Aug 15;82:466–477. doi: 10.1016/j.nbd.2015.08.008

Figure 3. Diminished TrkB activation in the striatum of zQ175 mice.

Figure 3

(A) Representative Western blot showing that total TrkB levels (both full-length and truncated TrkB) and phosphorylated TrkB (p-TrkB) levels are modestly reduced in the cortex of zQ175 mice at 12 months of age. (B) Total TrkB levels (both full-length and truncated TrkB) are not changed in the striatum of zQ175 mice, while p-TrkB level is significantly reduced in both 12-month-old zQ175 heterozygous and homozygous animals. (C, D) Densiometric quantification results of full-length TrkB (TrkB) and truncated TrkB (TrkB-T) protein levels in cortical (C) and striatal (D) lysates from wild type and zQ175 mice demonstrate no significant change of total TrkB levels in either the cortex or the striatum. (E) Quantification results of p-TrkB level in the cortex and the striatum of zQ175 mice. TrkB activation is only significantly diminished in the striatum but not the cortex. Values were normalized by total Erk1/2 levels and then averaged to compare with wild type.