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. 2016 Feb 3;9(2):113–117. doi: 10.14202/vetworld.2016.113-117

Table-1.

Primer sequences used to amplify HSP70 gene of Tharparkar cattle.

Fragment Primers Amplicon size Position on HSPA1A mRNA
I Forward 5′-AAACATGGCTATCGGCATCGACCT-3′ 295 181-475
Reverse 5′-AGGCTTGTCTCCGTCGTTGATGA-3′
II Forward 5′-CTAAGGTGCAGGTGAGCTACAAAG-3′ 220 474-693
Reverse 5′-TTGATGATCCTCAGCACGTTCAGC-3′

HSP70=Heat shock protein 70