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Details of primers used for PCR reaction.
| Primers | Sequence (5’-3’) | Target gene | Amplicon (bp) | References |
|---|---|---|---|---|
| stx1 (F) | CAG TTA ATG TGG TGG CGA AGG | stx1 | 348 | [22] |
| stx1 (R) | CAC CAG ACA ATG TAA CCG CTG | |||
| stx2 (F) | ATC CTA TTC CCG GGA GTT TAC G | stx2 | 584 | [22] |
| stx2 (R) | GCG TCA TCG TAT ACA CAG GAG C | |||
| eae (F) | TCA ATG CAG TTC CGT TAT CAG TT | eae | 482 | [20] |
| eae (R) | GTA AAG TCC GTT ACC CCA ACC TG |
PCR=Polymerase chain reaction, stx=Shiga toxin
