Table 1.

Details of primers and PCR conditions for amplification of OCT4A, OCT4 pseudogenes, and the internal control, GAPDH

Primer name	Primer sequence	Annealing temp	Amplicon size (bp)
OCT4-Pg1	F: 5´-CAT GCA RGC CCG AAA GAG AAA GCR AR-3´	60	107
	R: 5´-TGT GGC TGA TCT GCA GTG TGG G-3´
OCT4-Pg2	F: 5´-GTG TAC ATG TTT ATA AAG TTT GTG GTA GTG TTC-3´	58	380
	R: 5´-GGG TCG CTA GGT AAT TTT GTC ACT GG-3´
OCT4-Pg3	F: 5´-CTT CTC ACC CCC TCC AGG C-3´	62	841
	R: 5´-CCA CTG CTT GAT CGC TTG C-3´
OCT4-Pg4	F: 5´-GGG ACA CCT GGC TTC GGA TG-3´	56	172
	R: 5´-CCC CAC ACC TCA GAG CCT GA-3´
OCT4-Pg5	F: 5´-CAG TGA TTA TGC ACC ATG AGA GGA-3´	56	179
	R: 5´ GGG AAA GGC ACT AAG GAA CAC AG-3´
OCT4-Pg6	F: 5´-CCT AGC AAA ACC TCA ACG AGT CCC AG-3´	60	219
	R: 5´-CAA GAG GTT GTG GTG AGC GAA GG-3´
OCT4-Pg7	F: 5´-GCC AAA TTG CTG AAG CAG AAG GAT ATC-3´	58	347
	R: 5´-CTG GTC TTG TGA AAG GAT ACT CAG TGG-3´
OCT4A	F: 5-CTT CTC GCC CCC TCC AGG T-3´	65	495
	R: 5´-AAA TAG AAC CCC CAG GGT GAG C-3´
GAPDH	F: 5-GCCACATCGCTCAGACAC-3´	58	140
	R: 5´-GGCAACAATATCCACTTTACCAG-3´

PCR; Polymerase chain reaction.