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. 2016 Apr 4;18(1):7–12. doi: 10.22074/cellj.2016.3981

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Fig.3 — Evidence for interaction of SMAD3 transcripts with hsa-miR-590-5p. A. Down-regulation of SMAD3 expression following hsamiR- 590 overexpression detected through western analysis. Bands were quantitated using Total Lab Quant and results indicated 10% down-regulation of SMAD3 protein compared with the mock control and B. Luciferase assay validating SMAD3 as a target of hsa-miR- 590-5p. Luciferase activity was decreased in hsa-miR-590-5p transfected cells more than 15% compared with Hek293t cells co-transfected with mock, which supported direct interaction of hsa-miR-590-5p and the 3´-UTR of SMAD3 (*; P<0.05 vs. mock control).