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. Author manuscript; available in PMC: 2016 Apr 4.
Published in final edited form as: Acta Biomater. 2013 Apr 6;9(7):7420–7428. doi: 10.1016/j.actbio.2013.04.005

Fig. 5.

Fig. 5

Extracellular matrix production by human chondrocytes after 3 weeks of culture. Images where taken every 5 mm down the length of the gradient. (A) Whole mount Alcian Blue staining. Scale bar = 200 μm. (B) Sirius red staining of histological sections. Scale bar = 50 μm. (C) Type 2 collagen (green) and nuclear (blue) immunofluorescence of histological sections. Scale bar = 25 μm. (D) Sulfated gylcosaminoglycan biochemical quantification for week 3. (E) Hydroxyproline biochemical quantification for week 3. *P-value ≤ 0.05 relative to the 9.8 mM RGD concentration (45 mm gradient position).