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ADAP detects zeptomoles to attomoles of antibodies that bind antigens across a wide molecular weight distribution. The limits of ADAP detection for antibiotin, anti-insulin, anti-GFP, and antimouse IgG antibodies (antigen molecular weights of 0.24, 5.8, 27, and 150 kDa respectively) was determined by analyzing antibodies added into PBS or bovine serum. Error bars represent the standard deviation from triplicate samples.
