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. 2016 Feb 16;2(3):139–147. doi: 10.1021/acscentsci.5b00340

Figure 5.

Figure 5

Circumventing interference from anti-DNA autoantibodies by competition with free DNA. (a) Investigation of interference from anti-DNA autoantibodies. GFP–DNA conjugates were used to analyze anti-DNA-positive patient plasma and healthy normal plasma. Patient samples were grouped into those containing anti-single-stranded DNA antibodies (ssDNA) and those with anti-dsDNA antibodies (dsDNA). Interference was observed at dilution factors of 1 and 10 for all sample types (b) Competitor DNA was titrated into undiluted patient and normal plasma. The addition of competitor DNA eliminated background signal from interfering antibodies. (c) The experiment in (a) was repeated but with the addition of 100 μM competitor DNA which eliminated interference. (d) Purified GFP antibodies were added to anti-DNA positive and normal plasma. Detection of GFP antibodies was performed in the presence of 100 μM competitor DNA in all samples to confirm that it did not disrupt ADAP performance.