Fig. 1.

MMP14 and endoglin mRNA levels increase in endothelial cells after in vitro denudation. a HUVECs were wounded in vitro, leaving approximately 40 % intact of total monolayer. Photographs were taken at different times after wounding (0–8 h). Cells were lysed at the times indicated (0–24 h), and RNA was extracted and processed for qRT-PCR of human MMP14 and endoglin genes (b). KLF6, as a primary response gene to injury, was also quantified (c). mRNA expression levels of KLF6 (d), MMP14 (e) and endoglin (f) genes were measured upon KLF6 suppression with siRNA specific for KLF6 (siKLF6), using scrambled siRNA as a control. Fold change of mRNA expression levels with respect to basal conditions are indicated. Results were normalized to gene expression levels of 18S rRNA, as a housekeeping gene that does not change during the wound healing process. *p < 0.05; **p < 0.01; ***p < 0.001 with respect to basal conditions