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. 2016 Mar 31;57(3):1488–1499. doi: 10.1167/iovs.15-17994

Figure 1.

Figure 1

Müller glia mature slowly over the first postnatal month. (A–E) The Müller-specific marker CRALBP highlights morphologic maturation. (A) At P5, MG apicobasal processes are established at the inner and OLMs; insets show detail of the IPL, extending from the ILM to the INL. (B) At P10, MG extend side processes into the OPL (arrowhead) and define the boundary of the IPL (arrow). (C) At P15, MG display a marked increase in the density of processes: bands of MG fibers ensheathing neuronal processes in the IPL form, lamellar processes enveloping photoreceptor cell bodies are visible in the ONL. (D) At P25, Müller glial process in the IPL and ONL are well defined. (E) At P60, concomitant with slight decrease in retinal thickness, MG processes in IPL and ONL increase in density. (F–J) SOX2 stains MG and a subset of amacrine cells in the mature retina. (F) At P5, SOX2 is specifically expressed in the immature and elongated MG cell bodies in the INL (arrow) and a subpopulation of amacrine cells (arrowheads) in the INL. At P5, SOX2 expression is specifically colocalized with CRALBP expression (inset). (G) At P10, MG cell body morphology is mature and SOX2 staining is well defined. (G–J) SOX2 localization and expression in the INL remains static from P10 to P60. Scale bar: 50 μm.