Figure 5.

Sox2^MUTANT–Müller glial cell bodies are disorganized. (A–F) Müller glial cell bodies in the INL are specifically labeled with SOX9. (A, D) At P15, Sox2^MUTANT MG (D) have mislocalized cell bodies compared with Sox2^CONTROL (A), with some nuclei located ectopically in the OPL and the ONL (D, arrows). (B, E) At P25, Sox2^MUTANT–MG cell bodies (E, arrows) display increased disorganization compared with Sox2^CONTROL (B). Significant INL disorganization of Sox2^MUTANT–MG cell bodies was identified by a least squares regression analysis (P = 0.042, n = 5 eyes). (G) Exported and overlaid (x, y) coordinates of a representative P25 Sox2^MUTANT and Sox2^CONTROL pair with graphical representation of the organizational bands used in the analysis. (C, F) At P60, Sox2^MUTANT SOX9-positive MG remain disorganized (F, arrows) compared with Sox2^CONTROL (C), and are decreased in number compared to the P25 Sox2^MUTANT (E). Scale bar: 50 μm. Hoescht dye was used to stain the nuclei (blue).