Figure 7.

Lysosomal Accumulation of Tobacco Smoke Products in Alveolar Macrophages Compromises Migration to M. tuberculosis

(A) Representative images showing the characteristics of macrophages isolated by bronchoalveolar lavage from smokers and nonsmokers. Scale bars (left to right), 400, 10, 20, and 10 μm.

(B) Percentage of vacuolated macrophages was assessed in smokers, ex-smokers, and nonsmokers. Vacuolated macrophages were scored based on their autofluorescence and morphology.

(C) Number of macrophages that migrated through a transwell was assessed at 2 and 6 hr of incubation with either 0.1% fetal bovine serum (FBS) or Mtb H37Ra using macrophages from an ex-smoker. Values represent averages of a single experiment performed in triplicate.

(D) Number of macrophages from non/ex-smokers that migrated through a transwell toward Mtb H37Ra (assessed following 2 hr incubation).

(E) Fraction of macrophages that migrated in the transwell assay calculated from initial versus migrated macrophages of each morphology. Samples from smokers are split into vacuolated and normal with unique symbols for each patient.

Statistical significance was assessed by one-way ANOVA with Sidak’s post-test (B), Student’s t test (D), paired t test (E). See also Figure S6 and Table S4.