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Primer sequences for qRT-PCR
| Gene | Forward (5′→3′) | Reverse (3′→5′) |
|---|---|---|
| GAPDH | GAAGGTCGGTGTGAACGAAT | TTTGATGTTAGCGGGGTCTC |
| GDF9 | AACCCAGCAGAAGTCACCTC | AGGGGCTGAAGGAGGGAGG |
| AMH | GGGGAGACTGGAGAACAGC | AGAGCTCGGGGCTCCCATA |
AMH, anti-Müllerian hormone; GAPDH, glyceraldehyde-3-Phosphate Dehydrogenase; GDF, growth differentiation factor; qRT-PCR, quantitative reverse transcription-PCR.
