Fig. 5. Au–PLGA hybrid NPs produced by the in situ reduction method in w/o/w emulsion with SC. (b) Back scattered electron micrograph to show the location of Au NPs in the same area as (a). (c) NP size distribution of Au–PLGA hybrid NPs produced with STC and SC as surfactants (data obtained from TEM analysis). (d) HAADF-STEM micrograph of representative Au–PLGA hybrid NP produced with SC to show by Z-contrast the location of Au NPs inside representative Au–PLGA NPs. (e) HAADF-HR STEM image to show the crystal fringes of an Au NP located inside a PLGA NP. (f) HAADF-EDS analysis of the area selected in (d) to confirm the presence of a Au NP. (g) HAADF-STEM micrograph of a representative Au–PLGA hybrid NP produced with STC to show by Z-contrast the location of the single Au NPs entrapped inside a PLGA NP. (h) HAADF-HR STEM image of the Au NP depicted in figure (g) to show the crystal fringes of the Au NPs located inside the PLGA NPs. (i) HAADF-EDS analysis of the area selected in (g) to confirm the presence of Au NPs.