Table 3. UHPLC-MS solvent gradient for reverse phase analysis.
| Time (min) | Flow rate (μL min–1) | Mobile phase A (H2O%) | Mobile phase B (MeOH%) |
| 0 | 400 | 90 | 10 |
| 5 | 400 | 90 | 10 |
| 15 | 400 | 5 | 95 |
| 25 | 400 | 5 | 95 |
| 30 | 400 | 90 | 10 |
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