Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

letter

. 2016 Jan 8;590(1):3–12. doi: 10.1002/1873-3468.12037

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

Characterization of the interaction between syntenin and nectin‐1. (A) Co‐IPs of overexpressed wild‐type and truncated (deleted PDZ binding motif, ΔPDZBM) nectin‐1 and myc‐tagged syntenin in Cos cells. Syntenin was immunoprecipitated using anti‐syntenin1 antibody, and detected with the same antibody, and bound nectin was detected with an anti‐nectin antibody. Truncation of the PDZBM of nectin‐1 confers a loss of interaction. (B) Top: Schematic representation of C‐terminally Myc‐tagged full‐length syntenin and truncated constructs thereof that were used for co‐IPs of overexpressed nectin‐1. ‘Nt’ and ‘Ct’ indicate ‘N‐terminal region and C‐terminal region’. ‘+’ indicates that the co‐IPs of nectin‐1 and syntenin was positive with the indicated construct. Bottom: Co‐IP of nectin‐1 in and truncated C‐terminally Myc‐tagged syntenin constructs.