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Primer sequences for TR-qPCR
| Gene | Oligo name | Sequence (reads 5′–3′) |
|---|---|---|
| Pat | patF | ACGATCCATCTGTTAGGTTGCA |
| patR | CCATCCACCATGCTTGTATCCA | |
| Bar | barF | GCTCTACACCCACCTGCTG |
| barR | CAGCCCGATGACAGCGAC | |
| UBQ14 | UBQ14F | CAACGCTCCATCTTGTCCTT |
| UBQ14R | TGATCGTCTTTCCCGTAAGC | |
| PP2A | PP2A1F | CACTGCCCTGATTGAAAGTCAG |
| PP2A1R | GTCCAGAGCACGGATGTTATCT | |
| GAPDH | GAPDHF | TGATGCCAAGGCTGGAATTGCTT |
| GAPDHR | GTGTCGGATCAAGTCGATAACACGG |
GAPDH glyceraldehyde-3-phosphate dehydrogenase C subunit, PP2A protein phosphatase 2A, UBQ14 polyubiquitin
