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. 2016 Apr 1;98:150–157. doi: 10.1016/j.ymeth.2015.11.009

Fig. 4.

Fig. 4

Fluorescent beads as fiduciary markers for inter-channel alignment. The difference in direction and magnitude (B, C, D) of inter-channel misalignment within an image (A) is often dramatic and must be corrected. Microscope objectives attempt to correct for some of this, but residual values can be so great as to dominate the precision of the measurement.