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PCR Primers and Annealing Temperatures for Quantitation of Cytokine and Chemokine Transcript Levels
| Target | F/R | Sequence(5′ to 3′) | Tm (°C) | Product (bp) |
|---|---|---|---|---|
| IFN-γ | F | CCCCGCAGTATTGATGAGTT | 56 | 194 |
| R | TTGGAATAGTTGCCCGAGTC | |||
| CXCR3 | F | ACTACGATCAGCGCCTCAAT | 56 | 163 |
| R | CCTCTGGAGACCAGCAGAAC | |||
| CXCL10 | F | AAGTGCTGCCGTCATTTTCT | 56 | 186 |
| R | GTGGCAATGATCTCAACACG | |||
| IL-10 | F | CCAAGCCTTATCGGAAATGA | 56 | 162 |
| R | TTTTCACAGGGGAGAAATCG | |||
| β-actin | F | CACGATGGAGGGGCCGGACTCATC | 56 | 240 |
| R | TAAAGACCTCTATGCCAACACAGT |
F forward, R reverse
